J.I. Fritz* and D. Schneider
Universität für Bodenkultur Wien, Department IFA-Tulln, Konrad Lorenz Str. 20, 3430 Tulln, Austria
Phone: +43-2272-66280-559; FAX: +43-2272-66280-503; Email:
* Corresponding author.
The presented work focusses on methodology and screening for allelopathic effects in two European invaders and in one colour and medical plant. Himalayan Balsam (Impatiens glandulifera), Japanese Knotweed (Fallopia japonica) and Woad (Isatis tinctoria) were chosen and harvested as fresh plants during their growth period from places near Tulln, Austria. The search for bioactive compounds was not limited to those affecting higher plants (e.g. weeds), but we also screened the plants for effects against bacteria, fungi, algae and water flea. Advantages and limitations of the applied methods were discussed and procedures were optimised, if possible. Plant parts showing high activity were extracted and the solutions were applied to native soil. We monitored conventional microbiological parameters (e.g. CFU-counts) as well as changes of the microbial community structure (by molecular biological analysis) and degradation or bio-transformation of the introduced plant substances by HPLC-MS fingerprinting. This yielded an evaluated list of chemical analytical and biotest procedures, focussing on applied characterisation of interactions between secondary plant metabolites and soil microorganisms. Even thought it was not possible to identify degrading or passively influenced microorganisms from the treated soil, a methodology for further research in this field could be developed.
Keywords: allelochemicals, activity screening, biotests, soil micro-organism population, transformation, HPLC-MS analysis.
RECEIVED: April 23, 2015
ACCEPTED: May 11, 2015